IMPROVEMENT OF TRANSDUCTION EFFICIENCY OF RECOMBINANT ADENOASSOCIATEDVIRUS VECTOR BY ENTRAPMENT IN MULTILAMELLAR LIPOSOMES

Recombinant adeno-associated virus (AAV) has attracted considerable in terest as a potential vector for human gene therapy, but its transduct ion efficiency is quite low, The present study demonstrated AAV vector -associated liposomes to be more effective for in vitro gene transfer to human glioma cells than are liposomes containing plasmid DNA. Using vector-associated liposomes increased the transduction efficiency mor e than 10-fold compared to liposomes containing plasmid DNA and more t han 6-fold compared to AAV alone. From these results, AAV vector-assoc iated liposomes appear to be a good candidate for in vivo gene deliver y to human gliomas.