SIGNIFICANCE OF VASCULAR ENDOTHELIAL GROWTH-FACTOR MESSENGER-RNA EXPRESSION IN INVASION AND METASTASIS OF HEPATOCELLULAR-CARCINOMA

Vascular endothelial growth factor (VEGF) is an important mediator crf vascular development in tumors. We aimed at clarifying the relationsh ip between VEGF mRNA expression and invasion or metastasis of hepatoce llular carcinoma (HCC), Reverse transcript-polymerase chain reaction ( RT-PCR) was carried out on surgical specimens of 48 patients with HCC. The relative levels of VEGF mRNA expression were measured by determin ing a ratio between PCR products of VEGF and the endogenous internal s tandard gene p-actin. Expression of VEGF mRNA in tumor was found in 76 .5% (39/51) of patients, whereas it was 21.69% (11/51) in non tumorous liver. The levels of VEGF mRNA expression in tumors with tumorous emb oli and in poor-encapsulated tumors were higher than that without tumo rous emboli and in well-encapsulated tumors respectively (p<0.05 t tes t). There was no significance in the expression of VEGF mRNA between l arge:HCC (diameter>5cm) and small HCC (diameter less than or equal to 5cm) (p>0.05). VEGF may play an important role in the invasion and met astasis of HCC. Angiogenesis in tumor correlates with progression of H CC.