AN ENANTIOSELECTIVE FLUOROMETRIC ASSAY FOR ALCOHOL DEHYDROGENASES USING ALBUMIN-CATALYZED BETA-ELIMINATION OF UMBELLIFERONE

Authors
KLEIN G REYMOND JL
Citation
G. Klein et Jl. Reymond, AN ENANTIOSELECTIVE FLUOROMETRIC ASSAY FOR ALCOHOL DEHYDROGENASES USING ALBUMIN-CATALYZED BETA-ELIMINATION OF UMBELLIFERONE, Bioorganic & medicinal chemistry letters, 8(9), 1998, pp. 1113-1116
Citations number
20
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
8
Issue
9
Year of publication
1998
Pages
1113 - 1116
Database
ISI
SICI code
0960-894X(1998)8:9<1113:AEFAFA>2.0.ZU;2-J
Abstract
3-hydroxybutyl umbelliferyl ethers (R)-1 and (S)-1 are fluorogenic sub strates for alcohol dehydrogenases. Their oxidation forms ketone 2, wh ich undergoes beta-elimination of umbelliferone under catalysis by bov ine serum albumin, leading to a >20-fold fluorescence increase at lamb da(em) = 460 +/- 20 nm (lambda(ex) = 360 +/- 20 nm). Enantioselectivit y is determined in two separate tests with each enantiomeric substrate . (C) 1998 Elsevier Science Ltd. All rights reserved.