Nd. Machugh et al., CHARACTERIZATION OF A MONOCLONAL-ANTIBODY RECOGNIZING THE CD3-EPSILONCHAIN OF THE BOVINE T-CELL RECEPTOR COMPLEX, Veterinary immunology and immunopathology, 61(1), 1998, pp. 25-35
We describe the characterisation of a monoclonal antibody (mAb), desig
nated MM1A, which reacts with an antigen molecule on the surface of bo
vine alpha beta and gamma delta TcR+ T cells. The mAb immunoprecipitat
ed a series of polypeptides of 21 kDa, 22 kDa, 32 kDa, 36 kDa and 44 k
Da which is consistent with it recognising the TcR/CD3 complex. COS ce
lls, transfected with a cDNA encoding the bovine CD3 epsilon chain, re
acted with mAb MM1A indicating that the epitope recognised is on the e
psilon chain of the complex and confirming that the mAb recognised bov
ine CD3. (C) 1998 Elsevier Science B.V.