CHARACTERIZATION OF A MONOCLONAL-ANTIBODY RECOGNIZING THE CD3-EPSILONCHAIN OF THE BOVINE T-CELL RECEPTOR COMPLEX

Citation
Nd. Machugh et al., CHARACTERIZATION OF A MONOCLONAL-ANTIBODY RECOGNIZING THE CD3-EPSILONCHAIN OF THE BOVINE T-CELL RECEPTOR COMPLEX, Veterinary immunology and immunopathology, 61(1), 1998, pp. 25-35
Citations number
24
Categorie Soggetti
Immunology,"Veterinary Sciences
ISSN journal
01652427
Volume
61
Issue
1
Year of publication
1998
Pages
25 - 35
Database
ISI
SICI code
0165-2427(1998)61:1<25:COAMRT>2.0.ZU;2-S
Abstract
We describe the characterisation of a monoclonal antibody (mAb), desig nated MM1A, which reacts with an antigen molecule on the surface of bo vine alpha beta and gamma delta TcR+ T cells. The mAb immunoprecipitat ed a series of polypeptides of 21 kDa, 22 kDa, 32 kDa, 36 kDa and 44 k Da which is consistent with it recognising the TcR/CD3 complex. COS ce lls, transfected with a cDNA encoding the bovine CD3 epsilon chain, re acted with mAb MM1A indicating that the epitope recognised is on the e psilon chain of the complex and confirming that the mAb recognised bov ine CD3. (C) 1998 Elsevier Science B.V.