PLANT AMPHIPATHIC PROTEINS DELAY THE HYPERSENSITIVE RESPONSE CAUSED BY HARPIN(PSS) AND PSEUDOMONAS-SYRINGAE PV. SYRINGAE

Harpin(Pss) from the plant pathogen Pseudomonas syringae pv. syringae is a proteinaceous elicitor that induces a hypersensitive response (HR ) in non-host plants. The plant products which recognize harpin(Pss) i n the triggering of the HR are not yet known. According to the elicito r-receptor model, we hypothesize that an exogenous cell membrane recep tor infiltrated into the intercellular space will interfere with the i nteraction between harpin(Pss) and the putative receptor. We demonstra te a plant amphipathic protein (AP1) which can postpone the HR induced by harpin,,, as well as P. syringae pv. syringae. AP1 was extracted b y solubilizing proteins from healthy leaves in the nonpolar n-octanol buffer followed by a polar Tris buffer. The amphipathic extracts were then further separated by gel filtration and anion exchange chromatogr aphy to obtain highly purified AP1. Similar proteins can be extracted From cotton, tomato, and sweet pepper. The N-terminal amino acid seque nce of AP1 is conserved among cotton, tomato, and sweet pepper. The po stponement of the harpin(Pss)-mediated HR was characterized as a compe titive dosage-dependent pattern of AP1. An analysis of the bacterial p opulation development indicates that the effect of AP1 on the postpone ment of bacteria-mediated HR was attributed to the suppression of bact erial growth during the early stages of the HR. The time course analys is of the infiltration indicates that the postponement of HR resulted from the co-interaction between AP1 and the bacteria. Based on these r esults, we suggest that the postponement of bacteria-mediated HR is du e to the interference of the interaction between harpin(Pss) and the p utative receptor in the plant. Our research provides a new approach to elucidating the role that plants may play in the nonhost response cau sed by pathogens. (C) 1997 Academic Press Limited.