ISOLATION AND CHARACTERIZATION OF IS1416 FROM PSEUDOMONAS-GLUMAE, A NEW MEMBER OF THE IS3 FAMILY

Isolation and characterization of four different insertion sequence (I S) elements from Pseudomonas glumae MAFF 302744 through transposition into the entrapment vector pSH1063 are described. One of the elements, IS1416, was further characterized. IS1416 is 1322 bp long and carries 29-bp terminal inverted repeats flanked by a 3-bp direct duplication. IS1416 contains three open reading frames (ORFs), which are designate d ORFA1, ORFA2, and ORFB, on one strand. Both DNA sequence of IS1416 a nd the deduced amino acid sequences of its ORFs strongly suggest that IS1416 is a member of the IS3 family. and is closely related to IS401 from Pseudomonas cepacia and IS51 from Pseudomonas syringae. To our kn owledge, IS1416 is the first IS element isolated from P. glumae. The g ene organization and possible regulation of transposition functions of IS1416 are also discussed. (C) 1998 Academic Press.