ANALYSIS OF BACTERIOHOPANEPOLYOLS IN SEDIMENT AND BACTERIAL EXTRACTS BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY ATMOSPHERIC PRESSURE CHEMICAL-IONIZATION MASS-SPECTROMETRY
Pa. Fox et al., ANALYSIS OF BACTERIOHOPANEPOLYOLS IN SEDIMENT AND BACTERIAL EXTRACTS BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY ATMOSPHERIC PRESSURE CHEMICAL-IONIZATION MASS-SPECTROMETRY, Rapid communications in mass spectrometry, 12(10), 1998, pp. 609-612
We report the new application of negative ion atmospheric pressure che
mical ionization (APCI) to the detection of composite bacteriohopanepo
lyols in bacterial and sedimentary (or other natural environmental) sa
mples. The method employs the addition of dichloromethane to the high
performance liquid chromatography eluent prior to APCI to stimulate fo
rmation of chlorine addition/association ions from the hopanoid alcoho
ls. This approach provided the first direct analysis of composite bact
erial hopanoids in a sediment, identifying bacteriohopanetrol and bact
eriohopanetetrol cyclitol ether. (C) 1998 John Wiley & Sons, Ltd.