ANALYSIS OF BACTERIOHOPANEPOLYOLS IN SEDIMENT AND BACTERIAL EXTRACTS BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY ATMOSPHERIC PRESSURE CHEMICAL-IONIZATION MASS-SPECTROMETRY

Citation
Pa. Fox et al., ANALYSIS OF BACTERIOHOPANEPOLYOLS IN SEDIMENT AND BACTERIAL EXTRACTS BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY ATMOSPHERIC PRESSURE CHEMICAL-IONIZATION MASS-SPECTROMETRY, Rapid communications in mass spectrometry, 12(10), 1998, pp. 609-612
Citations number
9
Categorie Soggetti
Spectroscopy,"Chemistry Analytical
ISSN journal
09514198
Volume
12
Issue
10
Year of publication
1998
Pages
609 - 612
Database
ISI
SICI code
0951-4198(1998)12:10<609:AOBISA>2.0.ZU;2-J
Abstract
We report the new application of negative ion atmospheric pressure che mical ionization (APCI) to the detection of composite bacteriohopanepo lyols in bacterial and sedimentary (or other natural environmental) sa mples. The method employs the addition of dichloromethane to the high performance liquid chromatography eluent prior to APCI to stimulate fo rmation of chlorine addition/association ions from the hopanoid alcoho ls. This approach provided the first direct analysis of composite bact erial hopanoids in a sediment, identifying bacteriohopanetrol and bact eriohopanetetrol cyclitol ether. (C) 1998 John Wiley & Sons, Ltd.