THE EFFECTS OF FIBROBLAST GROWTH-FACTORS ON ISCHEMIC KIDNEY, LIVER AND GUT INJURIES

Objective. To explore the possibility of reducing reperfusion injuries of internal organ with acidic and basic fibroblast growth factors (aF GF and bFGF). Methods. Two kinds of ischemia and reperfusion animal mo dels were used in this study. In rat model of superior mesenteric arte ry (SMA) occlusion, microvascular clamp was placed on the root of SMA to cut off the blood flow for 45 minutes, and then the clamp was remov ed. In rat model of bilateral renal ischemia and reperfusion, both ren al arteries were clipped to get complete cessation of blood flow for 6 0 minutes, then the blood flow was allowed to return. At the onset of reperfusion, the doses of 4.0 mu g/rat of bFGF in SMA occluded rats or 2.6 mu g/rat of aFGF in rats with acute renal injury were administere d through the jugular vein. The liver and renal function examination, tissue bacterial study and histopathological evaluation were done to e valuate the treatment results. Results. The functional impairment of i schemic liver, gut and kidney were reduced with venous administration of aFGF or bFGF at the onset of reperfusion. The results of pathologic al and tissue bacterial examination supported the assertion of signifi cant protective effects of FGFs. Conclusions. The protective effects o f FGFs may come from the non-mitogenic effects of FGFs at the early an d the mitogenic effects at the late stage of tissue repair. These resu lts indicate a potential for clinical use of FGFs as a therapeutic mod ality in ischemic visceral organ injuries in the future.