CREATING MOSAICS IN DROSOPHILA

The ability to create mosaic animals allows the phenotypic analysis of patches of groups of genetically different cells that develop in a wi ld type environment. In Drosophila, a variety of techniques have been developed over the years to generate mosaics, and in this chapter, I r eview the techniques that our laboratory has developed. These include the ''Dominant Female Sterile'' technique which allows the analysis of gene functions to oogenesis and embryogenesis; the ''Gal4-UAS'' techn ique which allows the control of where and when specific genes are exp ressed; and, the ''Positive Marked Mutant Lineages'' technique which a llows clones of cells to express a specific reporter gene.