IMPLICATIONS OF THE SPATIAL AND TEMPORAL REGULATION OF HOX GENES ON DEVELOPMENT AND EVOLUTION

Nearly 20 years have passed since Ed Lewis revealed the importance of Hox genes in the specification of different segments in the anterior-p osterior axis of the fly. Pioneering studies by several authors, among others Garcia-Bellido and his student Gines Morata, helped to elabora te a theory of segmental specification that was strengthened with the arrival of molecular techniques to the field of Developmental Biology. The conservation of Hox genes in metazoans at the level of sequence, function and complex organization has resulted in the export of this D rosophila theory as a paradigm to interpret the development of axial s pecification in organisms less amenable to experimental study. There a re two main ways to interpret how Hox genes work in Drosophila. One co nsidering Hox genes as ''segment identity'' factors giving global prop erties to the segments in which they are active. Another considering H ox genes as encoding spatially restricted transcription factors requir ed for a number decisions taken at the cellular level. Here I use publ ished and unpublished experimental data to illustrate that early activ ation of the Hox genes does not establish a gene code that leads to '' segment identity''. I will stress the point that Hox expression patter ns develop with the embryo, that there are many genes involved in this modulation, and that the changing pattern of expression is important to achieve the final shape of the animal. I will show that, by interpr eting Hox gene function in this way, some apparently paradoxical resul ts in the Hox field can be answered. Finally, I discuss the implicatio ns of dynamic Hox gene expression on the evolution of segment morpholo gy.