CORRELATION BETWEEN POSITIVE SEROLOGY FOR PLASMODIUM-VIVAX-LIKE PLASMODIUM-SIMIOVALE MALARIA PARASITES IN THE HUMAN AND ANOPHELINE POPULATIONS IN THE STATE OF ACRE, BRAZIL

Antibodies against the Plasmodium vivax-like/P. simiovale malaria para site circumsporozoite repeat peptide (APGANQEGGAA)(3) were determined by enzyme-linked immunosorbent assay (ELISA) in 120 sera randomly coll ected in 1994 from adults in 3 localities of the malaria endemic area in the State of Acre, Brazil; antibody was detected in 18 (15%). A 'sa ndwich' ELISA using monoclonal antibody (mab) Pam 172, directed agains t the same peptide, was carried out on 1207 Anopheles oswaldoi 12 of w hich (1.0%) were positive, and 168 A. deaneorum, 2 of which (1.2%) wer e positive. This is the first report of serological detection of the P . vivax-like parasite in anophelines and the first report linking anop heline to human serology for this parasite in the same geographical ar ea. It is an additional indication that A. oswaldoi is a malaria vecto r in Acre.