EFFECTS OF LIPOXYGENASE METABOLITES OF ARACHIDONIC-ACID ON THE GROWTHOF HUMAN MONONUCLEAR MARROW-CELLS AND MARROW STROMAL CELL-CULTURES

THE effects of various lipoxygenase metabolites of arachidonic acid (A A) were investigated on the growth of freshly isolated human bone marr ow mononuclear cells and marrow stromal cell. cultures. LTB4, LXA(4), LXB4, 12-HETE and 15-HETE (1 mu M) decreased [H-3]-thymidine incorpora tion on marrow stromal cell cultures without affecting cell number. On ly 12-HETE showed a dose-response effect on [H-3]-thymidine incorporat ion. While LTB4 (1 mu M) decreased thymidine incorporation on marrow m ononuclear cells, LTC4, LXA(4), LXB4, 12-HETE and 15-HETE had no effec t. The lipoxygenase inhibitor NDGA had no effect on both cell types su ggesting no role of endogenous lipoxygenase metabolites on cell growth . These results suggest no important role of lipoxygenase metabolites of AA on the proliferation of human marrow mononuclear cells and marro w stromal cell cultures.