V. Desplat et al., EFFECTS OF LIPOXYGENASE METABOLITES OF ARACHIDONIC-ACID ON THE GROWTHOF HUMAN MONONUCLEAR MARROW-CELLS AND MARROW STROMAL CELL-CULTURES, Mediators of inflammation, 7(1), 1998, pp. 31-33
THE effects of various lipoxygenase metabolites of arachidonic acid (A
A) were investigated on the growth of freshly isolated human bone marr
ow mononuclear cells and marrow stromal cell. cultures. LTB4, LXA(4),
LXB4, 12-HETE and 15-HETE (1 mu M) decreased [H-3]-thymidine incorpora
tion on marrow stromal cell cultures without affecting cell number. On
ly 12-HETE showed a dose-response effect on [H-3]-thymidine incorporat
ion. While LTB4 (1 mu M) decreased thymidine incorporation on marrow m
ononuclear cells, LTC4, LXA(4), LXB4, 12-HETE and 15-HETE had no effec
t. The lipoxygenase inhibitor NDGA had no effect on both cell types su
ggesting no role of endogenous lipoxygenase metabolites on cell growth
. These results suggest no important role of lipoxygenase metabolites
of AA on the proliferation of human marrow mononuclear cells and marro
w stromal cell cultures.