IDENTIFICATION OF CARBOHYDRATES ON THE SURFACE-MEMBRANE OF PATHOGENICAND NONPATHOGENIC PISCINE HEMOFLAGELLATES, CRYPTOBIA-SALMOSITICA, CRYPTOBIA-BULLOCKI AND CRYPTOBIA-CATOSTOMI (KINETOPLASTIDA)
Sj. Feng et Ptk. Woo, IDENTIFICATION OF CARBOHYDRATES ON THE SURFACE-MEMBRANE OF PATHOGENICAND NONPATHOGENIC PISCINE HEMOFLAGELLATES, CRYPTOBIA-SALMOSITICA, CRYPTOBIA-BULLOCKI AND CRYPTOBIA-CATOSTOMI (KINETOPLASTIDA), Diseases of aquatic organisms, 32(3), 1998, pp. 201-209
Carbohydrates and protein glycoconjugates on the cell membranes of Cry
ptobia salmositica, C. bullocki and C. catostomi were analyzed using 1
3 highly purified lectins (unlabelled or digoxigenin/biotin labelled).
No agglutinations were observed with C. salmositica, C. bullocki and
C. catostomi using lectin TPA (Tetragonolobus prupureas agglutinin, fo
r alpha-L-fucose). C. salmositica was agglutinated by 3 of 12 lectins
[Con A, for alpha-man and alpha-D-glc; PSA, for alpha-man; PWM, for (g
lcNAc)3], while C. bullocki was agglutinated by 8 lectins and C. catos
tomi was agglutinated by 10 lectins. Glycoconjugate analysis with digo
xigenin or biotin labelled lectins showed a species-specific staining
pattern in pathogenic and nonpathogenic Cryptobia spp. The nonpathogen
ic C. catostomi had the strongest reaction. These results indicate tha
t the surface carbohydrate residues and glycoconjugate compositions on
Cryptobia spp. are different between species; they may be related to
the virulence of the parasite.