M. Schmidt et al., CHARACTERIZATION OF A DIRECTIONAL SELECTIVE INHIBITORY INPUT FROM THEMEDIAL TERMINAL NUCLEUS TO THE PRETECTAL NUCLEAR-COMPLEX IN THE RAT, European journal of neuroscience, 10(5), 1998, pp. 1533-1543
The receptive field properties of neurons in the medial terminal nucle
us of the accessory optic system (MTN) that project to the ipsilateral
nucleus of the optic tract (NOT) and dorsal terminal nucleus (DTN), a
s identified by antidromic electrical activation, were analysed in the
anaesthetized rat. The great majority (88%) of MTN neurons that were
antidromically activated from NOT and DTN preferred downward directed
movement of large visual stimuli while the remaining cells preferred u
pward directed stimulus movement. Distinct retrograde tracer injection
s into the NOT/DTN and the ipsilateral inferior olive (IO) revealed th
at no MTN neurons project to both targets. MTN neurons projecting to t
he ipsilateral NOT/DTN were predominantly found in the ventral part of
the MTN, whereas those projecting to the IO were found in the dorsal
part of the MTN. In situ hybridization for glutamic acid decarboxylase
(GAD) mRNA was used as a marker for GABAergic neurons. Up to 98% of M
TN neurons retrogradely labelled from the ipsilateral NOT/DTN also exp
ressed GAD mRNA. Earlier studies have shown that MTN neurons that pref
er upward directed stimulus movements are segregated from MTN neurons
that prefer downward directed stimulus movements. It also has been dem
onstrated that directionally selective neurons in the NOT/DTN prefer h
orizontal stimulus movements and receive an inhibitory input from ipsi
lateral MTN. Our results indicate that this input is mediated by GABAe
rgic cells in the ventral part of MTN, which to a large extent prefer
downward directed stimulus movements, and that the great majority of M
TN neurons that prefer upward directed stimulus movements project to o
ther targets one of which possibly is the IO.