POLYMERASE-CHAIN-REACTION TYPING OF D21S11 SHORT TANDEM REPEAT POLYMORPHISM BY CAPILLARY ELECTROPHORESIS - ALLELE FREQUENCIES AND SEQUENCING DATA IN A POPULATION-SAMPLE FROM CENTRAL ITALY

Blood samples were collected from 100 individuals living in Central It aly and analysed for STR locus D21S11 by capillary electrophoresis on an ABI Prism 310 genetic analyzer. For fragment sizing, PCR amplificat ion products, obtained using a 6-FAM 5'-labeled reverse primer and an unlabeled forward primer, were run with an internal size standard labe led with TAMRA dye and typed using the local reciprocal method. An all ele ladder consisting of a mix of sequenced amplified products was als o prepared. An Italian population database was established. No deviati on from Hardy-Weinberg equilibrium was observed. The results of statis tical analysis were highly informative (PD=0.94; mean exclusion change =0.66). DNA sequencing was performed on a set of representative allele s by Tag cycle sequencing using dye terminator labeling chemistry. A n ew structural variant was found. (C) 1998 Elsevier Science Ireland Ltd .