OPERATION OF MIXED-CULTURE IMMOBILIZED CELL REACTORS FOR THE METABOLISM OF META-NITROBENZOATE AND PARA-NITROBENZOATE BY COMAMONAS SP. JS46 AND COMAMONAS SP. JS47
Jl. Goodall et al., OPERATION OF MIXED-CULTURE IMMOBILIZED CELL REACTORS FOR THE METABOLISM OF META-NITROBENZOATE AND PARA-NITROBENZOATE BY COMAMONAS SP. JS46 AND COMAMONAS SP. JS47, Biotechnology and bioengineering, 59(1), 1998, pp. 21-27
The treatment of meta-and para-nitrobenzoic acid in an industrial wast
estream by Comamonas sp. JS46 and Comamonas sp. JS47 is investigated.
The most important feature of the wastestream is the constantly changi
ng concentration ratio of the two isomers. The most extreme occurrence
is considered here: the complete change in feed carbon source from on
e isomer to the other. A series of immobilized cell airlift reactor ex
periments are described to examine the operation and response of the s
ystem to these changes in the feed carbon source. Separate reactors co
ntaining each species immobilized are compared with a reactor containi
ng both species immobilized within the same bead, and to a reactor con
taining both species with each species confined to separate beads. On
the basis of response time necessary to recover the appropriate activi
ty, the reactor containing both species immobilized within the same be
ad offers the most effective arrangement. Interactions occurring betwe
en the two organisms in the coimmobilized system, mediated by the nitr
obenzoate metabolites, are discussed relative to the improved response
of this arrangement. (C) 1998 John Wiley & Sons, Inc.