In this paper,the most recent applications of high-performance size-ex
clusion chromatography (HPSEC) in analyses of fats and oils are presen
ted. Directly used in oils, the technique shows utility for evaluation
of polymers in used frying fats and oxidized samples, as well as for
analysis of low-caloric fats in blends. In concentrated fractions of p
olar compounds, characterization of virgin and refined oils and follow
-up of early oxidation stages are also attainable, An extended applica
tion to fatty acid derivatives allows complementary analyses of specif
ically oxidized and polymeric fatty acyl groups.