THE MULTISUBUNIT I-KAPPA-B KINASE COMPLEX SHOWS RANDOM SEQUENTIAL KINETICS AND IS ACTIVATED BY THE C-TERMINAL DOMAIN OF I-KAPPA-B-ALPHA

The multisubunit I kappa B kinase (IKK) catalyzes the signal-inducible phosphorylation of N-terminal serines of I kappa B, This phosphorylat ion is the key step in regulating the subsequent ubiquitination and pr oteolysis of I kappa B, which then releases NF-kappa B to promote gene transcription. As measured by P-33 incorporation into a GST-I kappa B alpha fusion protein, varying both the concentration of GST-I kappa B alpha and [gamma-P-33]ATP resulted in a kinetic pattern consistent wi th a random, sequential binding mechanism. Values of 55 nM and 7 mu M were obtained for the dissociation constants of GST-I kappa B alpha an d ATP, respectively. The value of alpha, a factor by which binding of one substrate changes the dissociation constant for the other substrat e, was determined to be 0.11. This indicates that the two substrates b ind in a cooperative fashion, Peptides corresponding to either amino a cids 26-42 (N-terminal peptide) or amino acids 279-303 (C-terminal pep tide) of I kappa B alpha inhibited the IKK-catalyzed phosphorylation o f GST-I kappa B alpha; the C-terminal peptide, unexpectedly, was more potent. The inhibition by the C-terminal peptide was competitive with respect to GST-I kappa B alpha and mixed with respect to ATP, which ve rified the sequential binding mechanism. The C-terminal peptide was al so a substrate for the enzyme, and a dissociation constant of 2.9-6.2 pw was obtained. Additionally, the N-terminal peptide was a substrate (K-m = 140 mu M), Competitive inhibition of the IKK-catalyzed phosphor ylation of the C-terminal peptide by the N-terminal peptide indicated that the peptides are phosphorylated by the same active site. Surprisi ngly, the presence of the C-terminal peptide greatly accelerated the r ate of phosphorylation of the N-terminal peptide as represented by a 1 60-fold increase in the apparent second-order rate constant (k(cat)/K- m), These results are consistent with an allosteric site present withi n IKK that recognizes the C terminus of I kappa B alpha and activates the enzyme. This previously unobserved interaction with the C terminus may represent an important mechanism by which the enzyme recognizes a nd phosphorylates I kappa B.