THE DETERMINATION OF CREATININE IN HUMAN URINE BY CAPILLARY-ZONE-ELECTROPHORESIS WITH PHOTODIODE-ARRAY DETECTION

The application of capillary zone electrophoresis (CZE) to the separat ion and determination of creatinine in human urine with photodiode arr ay detection was described. Optimum conditions for the separation were established. The effect of pH of the background buffer on the existen ce state of creatinine was investigated. Photodiode array detection pe rmitted the rapid identification of creatinine in the samples analysed . By selecting 234 Mn as monitoring wavelength, the interference from urea in urine was eliminated. By selecting a suitable pH, the interfer ences from other substances in urine were also eliminated. The linear range, detection limit, precision and recovery of the method were inve stigated.