HPLC DETERMINATION OF IDARUBICIN-ETOPOSIDE AND IDARUBICIN-ONDANSETRONMIXTURES IN 0.9-PERCENT SODIUM-CHLORIDE INJECTION USP

High performance liquid chromatographic methods have been developed fo r the assays of idarubicin-etoposide and idarubicin-ondansetron mixtur es in 0.9% sodium chloride injection USP. The separation and quantitat ion of the idarubicin-etoposide mixture was achieved on an octylsilane column at ambient temperature using a mobile phase of 73:27 v/v 0.02 M aqueous monobasic sodium phosphate - acetonitrile at a flow rate of 1.0 mL/min with UV detection at 254 nm. The separation was achieved wi thin 13 min. The method showed linearity for idarubicin hydrochloride and etoposide in the 2 - 32 and 10 - 160 mu g/mL, ranges, respectively . Accuracy and precision were in the 0.1 - 2.9% and 0.1 - 2.1% ranges, respectively, for both analytes. The separation and quantitation of t he idarubicin-ondansetron mixture was accomplished on an octadecylsila ne column at ambient temperature using a mobile phase of 57:43 v/v 0.0 2 M aqueous monobasic potassium phosphate - acetonitrile at a flow lat e of 1.8 mL/min with UV detection at 254 nm. The separation was achiev ed within 15 min. The method showed Linearity for idarubicin hydrochlo ride and ondansetron in the 2.4-38 and 2.3-37 mu g/mL, ranges, respect ively, for both analytes.