DETERMINATION OF A SYNTHETIC DECAPEPTIDE IN THE PLASMA OF MONKEYS ANDRATS BY NARROWBORE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Analytical methodology utilizing solid-phase extraction and reversed p hase narrowbore high-performance liquid chromatography is described fo r the specific determination of the decapeptide RWJ-47428-099 in the p lasma of monkeys and rats. Assay validation included,the demonstration of specificity, recoverability of added standard, stability, accuracy , and precision in both matrices. Linearity was demonstrated over the concentration range of 0.031 - 3.0 mu g/mL, in monkeys and 0.047 - 9.2 0 mu g/mL in rats. This methodology was subsequently implemented in su pport of preclinical drug metabolism investigations.