MAP INTEGRATION AT HUMAN-CHROMOSOME-10 - MOLECULAR AND CYTOGENETIC ANALYSIS OF A CHROMOSOME-SPECIFIC SOMATIC-CELL HYBRID PANEL AND GENOMIC CLONES, BASED ON A WELL-SUPPORTED GENETIC-MAP

Well-characterized, chromosome-specific somatic cell hybrid panels are powerful tools for the analysis of the human genome. We have characte rized a panel of human x hamster somatic cell hybrids retaining fragme nts of human chromosome 10 by fluorescence in situ hybridization and a ssociated them to genetic markers. Most of the hybrids were generated by the radiation-reduction method, starting from a chromosome 10-speci fic monochromosomal hybrid, whereas some were collected from hybrids r etaining chromosome 10-specific fragments as a result of spontaneous i n vitro rearrangements. PCR was used to score the retention of 57 micr osatellite markers evenly distributed along a well-supported framework genetic map containing 149 loci uniquely placed at 69 anchor points ( odds exceeding 1,000:1), with an average spacing of 2.8 cM. As an addi tional resource for genomic studies involving human chromosome 10, we report the cytogenetic localization of a series of YAC and PAC clones recognized by at least one genetic marker. Somatic cell hybrids provid e a powerful source of partial chromosome paints useful for detailed c linical cytogenetic and primate chromosome evolution investigations. F urthermore, correlation of the above physical, genetic, and cytogeneti c data contribute to an emerging consensus map of human chromosome 10.