The p53 tumor suppressor is activated in response to a variety of cell
ular stress signals, although specific in vivo signals that trigger tu
mor suppression are unknown. In mouse thymocytes, where p53 inactivati
on leads to tumorigenesis, several observations suggest that V(D)J rec
ombination of T-cell receptor (TCR) loci could provide a DNA damage si
gnal triggering p53-dependent apoptosis and tumor suppression. Inactiv
ation of p53 would allow V(D)J driven mutation of additional cancer ge
nes, facilitating tumorigenesis, Here, we show that mice with a p53 de
ficiency in thymocytes and unable to carry out V(D)J recombination are
not impaired in the development of thymoma, Recombination-activating
gene (RAG) deficiencies were introduced into both p53(-/-) mice and Tg
T Delta N transgenic mice, a strain in which 100% of the mice develop
thymoma due to thymocyte-specific inactivation of p53 by a simian viru
s 40 T-antigen variant. V(D)J recombination was dispensable for tumori
genesis since thymomas developed with or without the RAG-1 or RAG-2 ge
ne, although some delay was observed. When V(D)J recombination was sup
pressed by expression of rearranged TCR transgenes, 100% of the TgT De
lta N mice developed thymoma, surprisingly with reduced latency. Furth
er introduction of a RAG deficiency into these mice had no impact on t
he timing or frequency of tumorigenesis. Finally, karyotype and chromo
some painting analyses showed no evidence for TCR gene translocations
in p53-deficient thymomas, although abundant aneuploidy involving freq
uent duplication of certain chromosomes was present. Thus, contrary to
the current hypothesis, these studies indicate that signals other tha
n V(D)J recombination promote p53 tumor suppression in thymocytes and
that the mechanism of tumorigenesis is distinct from TCR translocation
oncogene activation.