Js. Perona et al., MOLECULAR PREDICTION OF RAT-LIVER TRIGLYCERIDES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of liquid chromatography & related technologies, 21(8), 1998, pp. 1185-1197
Citations number
28
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Triglyceride composition of rat liver was analyzed by HPLC with a non-
linear elution gradient of acetone in acetonitrile using a light-scatt
ering detector. Triglyceride molecular species were predicted by means
of simple and multiple linear regression analysis between the log k'
of the HPLC peaks and molecular variables, equivalent carbon number (E
CN) of the possible triglycerides, and chain length (CL) and number of
double bounds (DB) of each of the fatty acids in the glycerol molecul
e. The triglycerides presenting high random percentages were considere
d the principal molecular species in each HPLC peak. The main molecula
r species of rat liver triglycerides were palmitoyl-oleoyl-linoleoyl-g
lycerol, triolein, palmitoyl-dilinoleoyl-glycerol, dipalmitoyl-oleoyl-
glycerol and oleoyl-dilinoleoyl-glycerol. The systematics predicted mo
lecular species with arachidonic acid, such as dioleoyl-arachidonoyl-g
lycerol, dipalmitoyl-arachidonoyl-glycerol, and oleoyl-linoleoayl-arac
hidonoyl-glycerol, and with docosahexaenoic acid, including dipalmitoy
l-dacosahexaenoyl-glycerol, palmitoyl-oleoyl-docosahexaenoyl-glycerol,
and palmitoyl-linoleoyl-docosahexaenoyl-glycerol.