SELENIUM REGULATES GENE-EXPRESSION FOR ESTROGEN SULFOTRANSFERASE AND ALPHA-2U-GLOBULIN IN RAT-LIVER

Dietary intake of the essential trace element selenium (Se) regulates expression of genes for selenoproteins and certain non-Se-containing p roteins. However, these proteins do not account for all of Se's biolog ical effects. The objective of this work was to identify additional ge nes whose expression is regulated by Se. Identification of these genes may reveal new functions for Se or define mechanisms for its biologic al effects. Weanling male Sprague-Dawley rats were fed a Torula yeast- based Se-deficient basal diet or the same diet supplemented with 0.5 m g Se/kg diet as sodium selenite for 13 weeks. Total RNA was used as te mplate for RNA fingerprinting. Two differentially expressed cDNA fragm ents were identified and cloned. The first had 99% nucleotide identity with rat Liver estrogen sulfotransferase (EST) isoform-6. The second had 99% nucleotide sequence identity with rat liver alpha 2u-globulin. The mRNA levels for both were markedly reduced in Se deficiency. Lase r densitometry showed that EST mRNA in Se deficiency was 7.3% of that in Se-adequate rat liver. The level of ct Zu-globulin mRNA in Se-defic ient rat liver was only 12.6% of that in Se-adequate rat liver. These results indicate that dietary Se may play a role in steroid hormone me tabolism in rat Liver. (C) 1998 Elsevier Science Ltd. All rights reser ved.