DIAGNOSIS OF CYTOMEGALOVIRUS-INFECTION IN KIDNEY-TRANSPLANT RECIPIENTS BY A QUANTITATIVE RNA-DNA HYBRID CAPTURE ASSAY FOR CYTOMEGALOVIRUS DNA IN LEUKOCYTES

Citation
H. Rollag et al., DIAGNOSIS OF CYTOMEGALOVIRUS-INFECTION IN KIDNEY-TRANSPLANT RECIPIENTS BY A QUANTITATIVE RNA-DNA HYBRID CAPTURE ASSAY FOR CYTOMEGALOVIRUS DNA IN LEUKOCYTES, European journal of clinical microbiology & infectious diseases, 17(2), 1998, pp. 124-127
Citations number
10
Categorie Soggetti
Microbiology,"Infectious Diseases
ISSN journal
09349723
Volume
17
Issue
2
Year of publication
1998
Pages
124 - 127
Database
ISI
SICI code
0934-9723(1998)17:2<124:DOCIKR>2.0.ZU;2-E
Abstract
The clinical value of a new RNA-DNA hybridization assay for quantifica tion of cytomegalovirus (CMV) DNA in leukocytes [Hybrid Capture CMV DN A Assay (HCA); Murex Biotech? UK] was evaluated. The PICA was compared with an assay for CMV pp65 antigen in leukocytes and an in-house CMV polymerase chain reaction PCR (CMV-PCR) on parallel blood samples. The PICA and the CMV-PCR were less sensitive than the CMV pp65 assay, but the positive predictive value of all three methods for CMV disease wa s 50% or less. However, when quantitation of viral load by HCA and CMV pp65 assay was taken into consideration, both assays were superior to CMV-PCR in predicting CMV disease.