DIAGNOSIS OF CYTOMEGALOVIRUS-INFECTION IN KIDNEY-TRANSPLANT RECIPIENTS BY A QUANTITATIVE RNA-DNA HYBRID CAPTURE ASSAY FOR CYTOMEGALOVIRUS DNA IN LEUKOCYTES
H. Rollag et al., DIAGNOSIS OF CYTOMEGALOVIRUS-INFECTION IN KIDNEY-TRANSPLANT RECIPIENTS BY A QUANTITATIVE RNA-DNA HYBRID CAPTURE ASSAY FOR CYTOMEGALOVIRUS DNA IN LEUKOCYTES, European journal of clinical microbiology & infectious diseases, 17(2), 1998, pp. 124-127
The clinical value of a new RNA-DNA hybridization assay for quantifica
tion of cytomegalovirus (CMV) DNA in leukocytes [Hybrid Capture CMV DN
A Assay (HCA); Murex Biotech? UK] was evaluated. The PICA was compared
with an assay for CMV pp65 antigen in leukocytes and an in-house CMV
polymerase chain reaction PCR (CMV-PCR) on parallel blood samples. The
PICA and the CMV-PCR were less sensitive than the CMV pp65 assay, but
the positive predictive value of all three methods for CMV disease wa
s 50% or less. However, when quantitation of viral load by HCA and CMV
pp65 assay was taken into consideration, both assays were superior to
CMV-PCR in predicting CMV disease.