DETERMINATION OF THE PARENT OF ORIGIN IN 9 CASES OF PRENATALLY DETECTED CHROMOSOME-ABERRATIONS FOUND AFTER INTRACYTOPLASMIC SPERM INJECTION

Prenatal cytogenetic analysis of 71 fetuses conceived by intracytoplas mic sperm injection (ICSI) resulted in the detection of nine (12.7%) c hromosome aberrations including two cases of 47,XXY, four cases involv ing a 45,X cell line and three autosomal trisomies, Molecular analysis of the parental origin of the deleted or supernumerary chromosome was performed by using polymorphic microsatellite markers, Six cases invo lving a sex chromosome abnormality were found to be of paternal origin while the two trisomic cases that could be analysed were of maternal origin, Two cases involved the same infertile couple who had two conse cutive ICSI pregnancies terminated because of a chromosome abnormality . The replaced embryos in both cases originated from a single batch of ICSI fertilized oocytes of which part was used to initiate the first pregnancy and part was cryopreserved and used to initiate the second p regnancy.