ESTIMATION OF THE PARTITIONING CHARACTERISTICS OF DRUGS - A COMPARISON OF A LARGE AND DIVERSE DRUG SERIES UTILIZING CHROMATOGRAPHIC AND ELECTROPHORETIC METHODOLOGY

1-Octanol-water log P values for a large number of standards and bioac tive molecules have been correlated to the logarithm of the correspond ing capacity factors determined by reversed-phase high-performance liq uid chromatography, using a novel dynamically coated phase, containing phosphatidylcholine. Similarly a correlation was also obtained for lo g P and capacity factors determined by micellar electrokinetic capilla ry chromatography (MECC), involving the use of phosphatidylcholine - b ile acid mixed micelles in the separation buffer. Statistical analysis of data obtained via both methods has shown that either method will g ive reliable log P predictions, although MECC is generally more useful for neutral and basic compounds. It is recommended that, as both meth ods can easily be set up in an analytical laboratory, their combined u se provides rapid methodology for the confident estimation of hydropho bicity, as measured by log P for the widest diversity of chemical stru ctures.