HUMAN-PAPILLOMAVIRUS TYPE-33 DNA AND E6-E7 TRANSCRIPTS IN LATE PASSAGES OF THE UT-DEC-1 VAGINAL KERATINOCYTE CELL-LINE

Transcription of human papillomavirus (HPV) type 33 early region was a nalysed in the UT-DEC-1 keratinocyte cell line, which has been derived from a HPV-33-containing mild vaginal dysplasia. Fifteen cDNA clones from transcripts from the E6-E7 open reading frames were constructed a nd analysed. Most clones represented viral transcripts spliced within the E6 open reading frame, probably encoding the E7 protein. Interesti ngly, a less abundant unspliced transcript species with coding capacit y for the full length E6 protein was found, reported here for the firs t time for the malignancy-associated HPV type 33. (C) 1998 Academic Pr ess Limited.