STRUCTURE OF LYSOPHOSPHATIDYLGLYCERYL-REMAZOLBRILLIANT BLUE (LPG-RBB)FROM THE SURFACE OF DYED WHEAT-STARCH GRANULES

Wheat starch granule surface was covalently stained with Remazol-brill iant blue-R dye (RBB) and then extracted with 1% SDS containing 1% 2-m ercaptoethanol (2-ME) at room temperature for 14.5 hr. The extracted b lue-staining material (A(650)) separated into two fractions. Low molec ular weight (LMW) material was further purified by Sephadex G-75 size- exclusion chromatography and thin-layer chromatography. infrared and n uclear magnetic resonance (H-1-NMR and C-13-NMR) spectroscopy indicate d that the structure of the purified LMW material was 18-O-(6-lysophos phatidylglyceryl)-RBB.