16S RIBOSOMAL-RNA SEQUENCE DIVERSITY IN MYCOBACTERIUM-CELATUM STRAINSCAUSED BY PRESENCE OF 2 DIFFERENT COPIES OF 16S RIBOSOMAL-RNA GENE

Authors
REISCHL U FELDMANN K NAUMANN L GAUGLER BJM NINET B HIRSCHEL B EMLER S
Citation
U. Reischl et al., 16S RIBOSOMAL-RNA SEQUENCE DIVERSITY IN MYCOBACTERIUM-CELATUM STRAINSCAUSED BY PRESENCE OF 2 DIFFERENT COPIES OF 16S RIBOSOMAL-RNA GENE, Journal of clinical microbiology, 36(6), 1998, pp. 1761-1764
Citations number
27
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
36
Issue
6
Year of publication
1998
Pages
1761 - 1764
Database
ISI
SICI code
0095-1137(1998)36:6<1761:1RSDIM>2.0.ZU;2-P
Abstract
Direct sequencing of the 16S rRNA gene (16S rDNA) of Mycobacterium cel atum isolates showed ambiguities, suggesting heterogeneity. Cloned 16S rDNA yielded two copies of the gene, which differed by insertion of a thymine at position 214 and by additional mismatches. Restriction fra gment length polymorphism analysis confirmed the presence of two copie s of 16S rDNA within the bacterial chromosome.