STABILIZED, FREEZE-DRIED PCR MIX FOR DETECTION OF MYCOBACTERIA

Authors
KLATSER PR KUIJPER S VANINGEN CW KOLK AHJ
Citation
Pr. Klatser et al., STABILIZED, FREEZE-DRIED PCR MIX FOR DETECTION OF MYCOBACTERIA, Journal of clinical microbiology, 36(6), 1998, pp. 1798-1800
Citations number
6
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
36
Issue
6
Year of publication
1998
Pages
1798 - 1800
Database
ISI
SICI code
0095-1137(1998)36:6<1798:SFPMFD>2.0.ZU;2-O
Abstract
We report here the development of a freeze-drying procedure allowing s tabilization at ambient temperature of preoptimized, premixed, and pre dispensed PCR mixes aimed at the detection of mycobacteria in clinical materials. The freeze-dried mixes retained activity at 4 degrees C an d at 20 degrees C for 1 gear and for 3 months at 37 degrees C, as judg ed by their performance with 50 and 500 fg of purified Mycobacterium b ovis BCG target DNA.