AN EXPERIMENTAL SELECTION SYSTEM TO IDENTIFY BACTERIAL-CELLS EXHIBITING A NEW DNA HOST-SPECIFICITY

Authors
KUNZ A MEISEL A MACKELDANZ P REUTER M KRUGER DH
Citation
A. Kunz et al., AN EXPERIMENTAL SELECTION SYSTEM TO IDENTIFY BACTERIAL-CELLS EXHIBITING A NEW DNA HOST-SPECIFICITY, Biological chemistry, 379(4-5), 1998, pp. 563-566
Citations number
11
Categorie Soggetti
Biology
Journal title
Biological chemistry → ACNP
ISSN journal
14316730
Volume
379
Issue
4-5
Year of publication
1998
Pages
563 - 566
Database
ISI
SICI code
1431-6730(1998)379:4-5<563:AESSTI>2.0.ZU;2-V
Abstract
Restriction-modification enzymes interact with DNA sequences in a high ly specific manner. Mutations within the DNA binding region of the enz ymes could be expected to produce enzyme variants with changed DNA seq uence specificities. We developed an efficient in vivo selection syste m that enabled us to detect one cell coding for a restriction-modifica tion system with a new DNA sequence specificity in a background of mor e than 10(6) cells with the original DNA sequence specificity.