CAPILLARY ISOTACHOPHORESIS WITH FIBEROPTIC RAMAN-SPECTROSCOPIC DETECTION - PERFORMANCE AND APPLICATION TO RIBONUCLEOTIDES

A fiber-optic Raman probe fitted with a microscope objective was used to obtain on-line normal Raman spectra of adenosine 5'-monophosphate, cytidine 5'-monophosphate, guanosine 5'-monophosphate and uridine 5'-m onophosphate separated by capillary isotachophoresis. With multimode o ptical fiber, the system interrograted a 40-mu m length of capillary. Fiber-optic coupling facilitated use of an unmodified spectrograph and conventional capillary mounting systems. Raman spectra were excited w ith a 2W 532 nm NdYVO4, laser as the excitation source, with collectio n of 1 spectrum per second. Even at 2.10(-5) M initial concentration, Raman spectra were obtained at a good signal-to-noise ratio. (C) 1998 Elsevier Science B.V.