SEX IDENTIFICATION OF ELK (CERVUS-ELAPHUS CANADENSIS), MOOSE (ALCES-ALCES), AND WHITE-TAILED DEER (ODOCOILEUS-VIRGINIANUS) USING THE POLYMERASE-CHAIN-REACTION
Pj. Wilson et Bn. White, SEX IDENTIFICATION OF ELK (CERVUS-ELAPHUS CANADENSIS), MOOSE (ALCES-ALCES), AND WHITE-TAILED DEER (ODOCOILEUS-VIRGINIANUS) USING THE POLYMERASE-CHAIN-REACTION, Journal of forensic sciences, 43(3), 1998, pp. 477-482
We have developed a PCR-based protocol to determine the gender of tiss
ue samples originating from elk (Cervus elephus canadensis), moose (Al
ces alces) and white-tailed deer (Odocoileus virginianus). The techniq
ue simultaneously amplifies a conserved region of the sex-determining
gene on the Y-chromosome (Sry) and a region of the Fragile X mental re
tardation gene (Fmr-1). The multiplex nature of this protocol allows t
he determination of gender using the Sry marker with the Fmr-1 marker
providing an internal control. This technique is applicable to the enf
orcement of the validation tag system for game species. Data are provi
ded from a wildlife investigation in Ontario.