SEX IDENTIFICATION OF ELK (CERVUS-ELAPHUS CANADENSIS), MOOSE (ALCES-ALCES), AND WHITE-TAILED DEER (ODOCOILEUS-VIRGINIANUS) USING THE POLYMERASE-CHAIN-REACTION

Citation
Pj. Wilson et Bn. White, SEX IDENTIFICATION OF ELK (CERVUS-ELAPHUS CANADENSIS), MOOSE (ALCES-ALCES), AND WHITE-TAILED DEER (ODOCOILEUS-VIRGINIANUS) USING THE POLYMERASE-CHAIN-REACTION, Journal of forensic sciences, 43(3), 1998, pp. 477-482
Citations number
16
Categorie Soggetti
Medicine, Legal
ISSN journal
00221198
Volume
43
Issue
3
Year of publication
1998
Pages
477 - 482
Database
ISI
SICI code
0022-1198(1998)43:3<477:SIOE(C>2.0.ZU;2-K
Abstract
We have developed a PCR-based protocol to determine the gender of tiss ue samples originating from elk (Cervus elephus canadensis), moose (Al ces alces) and white-tailed deer (Odocoileus virginianus). The techniq ue simultaneously amplifies a conserved region of the sex-determining gene on the Y-chromosome (Sry) and a region of the Fragile X mental re tardation gene (Fmr-1). The multiplex nature of this protocol allows t he determination of gender using the Sry marker with the Fmr-1 marker providing an internal control. This technique is applicable to the enf orcement of the validation tag system for game species. Data are provi ded from a wildlife investigation in Ontario.