SIMULTANEOUS GENOTYPING OF ALCOHOL-DEHYDROGENASE-2 (ADH2) AND ALDEHYDE-DEHYDROGENASE-2 (ALDH2) LOCI BY AMPLIFIED PRODUCT LENGTH POLYMORPHISM (APLP) ANALYSIS

Authors
AOSHIMA T UMETSU K YUASA I WATANABE G SUZUKI T
Citation
T. Aoshima et al., SIMULTANEOUS GENOTYPING OF ALCOHOL-DEHYDROGENASE-2 (ADH2) AND ALDEHYDE-DEHYDROGENASE-2 (ALDH2) LOCI BY AMPLIFIED PRODUCT LENGTH POLYMORPHISM (APLP) ANALYSIS, Electrophoresis, 19(5), 1998, pp. 659-660
Citations number
18
Categorie Soggetti
Biochemical Research Methods","Chemistry Analytical
Journal title
Electrophoresis → ACNP
ISSN journal
01730835
Volume
19
Issue
5
Year of publication
1998
Pages
659 - 660
Database
ISI
SICI code
0173-0835(1998)19:5<659:SGOA(A>2.0.ZU;2-G
Abstract
Genotyping of the alcohol dehydrogenase 2 (ADH2) and aldehyde dehydrog enase 2 (ALDH2) loci is important in alcohol studies. We describe a me thod for simulataneous genotyping of ADH2 and ALDH2 based on amplified product length polymorphism (APLP) analysis. Two polymerase chain rea ction (PCR) fragments for ADH2 (57 bp, 53 bp) and two for ALDH2 (78 bp , 73 bp) are simultaneously amplified. Nine banding patterns reflectin g the genotypes of the ADH2 and ALDH2 loci are clearly and unambiguous ly distinguished. The APLP method seems to be particularly suited for large-scale population studies of ADH2 and ALDH2 loci because it is si mple and rapid.