DEVELOPMENT OF A 2-DIMENSIONAL GEL-ELECTROPHORESIS DATABASE OF HUMAN LYSOSOMAL PROTEINS

Two-dimensional gel electrophoresis databases have been generated for a range of tissue cell and fluid types, from a number of species. A ma jor difficulty in the development of such databases is the large numbe r of proteins present in even a single cell type (> 10 000) and the lo w levels of many of these proteins. One approach to overcome these dif ficulties is to fractionate the cell into its organelles and generate individual databases for each subcellular component. This has the adde d advantage of assigning a cellular location to each protein identifie d. Here we report the development of a two-dimensional gel electrophor esis database of lysosomal proteins.