BONE MORPHOGENETIC PROTEIN IN BONE NEOPLASMS - COMPARISON OF DIFFERENT DETECTION METHODS

The presence of bone morphogenetic proteins (BMPs) in 13 primary bone neoplasms was investigated with conventional bioassay method and immun ohistochemically using antiserum against highly purified mixture of bo vine BMPs as antibody. In conventional bioassay after implantation of lyophilized bone tumor tissues into mouse muscle pouches 9/13 samples turned out positive by radiography and 10/13 histologically. By immuno histochemical staining, using the avidin-biotin-peroxidase method, sig ns of BMPs could be verified in all cases investigated. Microscopical scoring Showed the local concentration of BMPs to be especially high i n sections from giant cell tumors when compared to other bone neoplasm s.