Ma. Zambre et al., PLANT-REGENERATION FROM EMBRYO-DERIVED CALLUS IN PHASEOLUS-VULGARIS L. (COMMON BEAN) AND PHASEOLUS-ACUTIFOLIUS GRAY,A (TEPARY BEAN), Plant cell reports, 17(8), 1998, pp. 626-630
Regeneration-competent callus of Phaseolus vulgaris and P. acutifolius
was obtained from mature embryo explants on a medium containing thidi
azuron and indole-3-acetic acid. For the P. vulgaris genotype Xan-159,
regeneration was achieved from cotyledon explants, but not from embry
onic axis explants. Both explants could be used for the P. acutifolius
genotype NI 574 but embryonic axes gave the best results. In-vitro-ro
oted plantlets of P. acutifolius could readily be established in the g
reenhouse. For P. vulgaris hardening problems with in-vitro-rooted pla
ntlets could be overcome by means of in vitro grafting. The potential
of the described procedure for ob raining transgenic P. vulgaris plant
s is discussed.