Ks. Sarma et Smd. Rogers, PLANT-REGENERATION AND MULTIPLICATION OF THE EMERGENT WETLAND MONOCOTJUNCUS-ACCUMINATUS, Plant cell reports, 17(8), 1998, pp. 656-660
A reliable callus regeneration and shoot multiplication system for wet
land monocot Juncus accuminatus has been established. Callus was induc
ed from 6-day-old seedlings on Murashige and Skoog medium supplemented
with 5 mg/l picloram. The callus differentiated into shoots upon tran
sfer to 5 mg/l benzyladenine (BA)-supplemented medium. Effects of medi
um pH (3.8-7.8) and source of callus (grown in the dark or continuous
light) on regeneration were determined. Both parameters significantly
influenced regeneration. Regenerated shoots were multiplied by subcult
uring shoots onto 5 mg/l BA medium at 4-week intervals. The regenerate
d shoots were rooted on 0.1 mg/l naphthaleneacetic-acid-supplemented m
edium. The rooted plants were transferred to pots containing a commerc
ial potting mix and established in the greenhouse. Plants covered with
plastic grew faster and flowered earlier than uncovered plants. All p
lants set viable seeds.