MIGRATION INDUCTION OF CONTACT INHIBITED C3H 10T1 2 CELLS BY PROTEIN-KINASE-C (PKC) DEPENDENT PROCESS/

Treatment of confluent contact inhibited 10T1/2 cells with TPA or OAG induced a dramatic increase of the number of migrating cells, on cover slides inserted into culture dishes. When cover slides were coated wi th collagen IV or fibronectin, there was a similar increase of the num ber of migrating cells. RT PCR showed the presence of alpha PKC gene t ranscripts and the lack of beta and gamma PKC. Western blot analysis s howed translocation of 80 kD alpha PKC to membranous fraction followin g brief treatment with TPA, and down-regulation of PKC after longer ex posure to TPA. Collagen IV and fibronectin treatment of 10T1/2 cells i nduced MAP kinase, (MEK) kinase in the presence and in absence of FCS. Signal transduction pathway depending on protein kinase C and integri n receptors activation appears to facilitate migration of 10T1/2 cells and may be involved in the mechanism of the escape from contact inhib ition of movement.