E. Bilsland et al., GENOMIC DISRUPTION OF 6 BUDDING YEAST GENES GIVES ONE DRASTIC EXAMPLEOF PHENOTYPE STRAIN-DEPENDENCE, Yeast, 14(7), 1998, pp. 655-664
Using PCR to construct disruption cassettes, null alleles of six genes
have been created in Saccharomyces cerevisiae. In a FY1679 background
, no defects were detected in any of the haploid deletion mutants with
respect to growth, gross morphology, or mating. A diploid Fy1679-deri
ved Delta ygl194c/Delta ygl194c homozygous disruptant displayed reduce
d sporulation. In contrast to the lack of phenotypic consequences of D
elta yol100w disruptions in the FY1679 background, in the CEN.PK2 stra
in even a heterozygous disruption of the same gene caused striking eff
ects, very slow vegetative growth and highly impaired sporulation. Tet
rad analysis showed YOL100w to be an essential gene in this strain. A
copy of the YGL194c or the YOL100w wild-type gene borne on a centromer
ic episomal plasmid was introduced into a corresponding disruption mut
ant strain, and in both cases was found to partially complement the de
fects. (C) 1998 John Wiley & Sons, Ltd.