DELAYED-TYPE HYPERSENSITIVITY THEILERIN TEST IN CATTLE VACCINATED AGAINST THEILERIA-ANNULATA INFECTION

Theilerin was prepared and its total nitrogen was determined at level of 1 mg/ml. Seventy-two Holstein Friesian cattle recovered from acute theileriosis or vaccinated received 0.1 ml of theilerin intradermally at normal concentration of total nitrogen or other dilutions (1/10 and 1/100). Some 60%, of calves and 66.67% of milking cows receiving norm al theilerin showed more than 1 mm increase in skin thickness at the s ite of inoculation (mean value of 1.86 mm for calves and 3.46 mm for m ilking cows). The pathological examination of the inoculation site sho wed infiltration of lymphocytes. No changes of general condition were observed in animals under study. The result indicated that the test is positive and could be used for checking Immunity in vaccinated animal s. (C) 1998 Elsevier Science B.V.