MONOCLONAL-ANTIBODIES AGAINST TEA POLYPHENOLS - A NOVEL IMMUNOASSAY TO DETECT POLYPHENOLS IN BIOLOGICAL-FLUIDS

Authors
GANI M MCGUINNESS BJ DAVIES AP
Citation
M. Gani et al., MONOCLONAL-ANTIBODIES AGAINST TEA POLYPHENOLS - A NOVEL IMMUNOASSAY TO DETECT POLYPHENOLS IN BIOLOGICAL-FLUIDS, FOOD AND AGRICULTURAL IMMUNOLOGY, 10(1), 1998, pp. 13-22
Citations number
10
Categorie Soggetti
Food Science & Tenology","Chemistry Applied",Toxicology,Immunology,"Agriculture Dairy & AnumalScience
Journal title
FOOD AND AGRICULTURAL IMMUNOLOGY → ACNP
ISSN journal
09540105
Volume
10
Issue
1
Year of publication
1998
Pages
13 - 22
Database
ISI
SICI code
0954-0105(1998)10:1<13:MATP-A>2.0.ZU;2-Y
Abstract
A panel of monoclonal antibodies (MAbs) was raised against tea polyphe nols conjugated to bovine serum albumin. A cross-reactivity study carr ied out against various purified polyphenols showed that most of the a ntibodies generated have the ability to recognize epigallocatechin > e picatechin > thearubigin = theaflavin > epicatechin-3-gallate = epigal locatechin-3-gallate > rutin. One of the selected MAbs, 5756.3, was us ed in an immunoassay to measure polyphenols in tea, urine and blood sa mples.