EMBRYOGENIC MANGO CULTURES SELECTED FOR RESISTANCE TO COLLETOTRICHUM-GLOEOSPORIOIDES CULTURE FILTRATE SHOW VARIATION IN RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS

Genomic DNA isolated from embryogenic cultures of two mango cultivars, 'Hindi' and 'Carabao,' that had been selected for resistance to the c ulture filtrate of Colletotrichum gloeosporioides, was analyzed using Randomly Amplified Polymorphic DNA (RAPD). In vitro selection caused c hanges in RAPD markers in the selected embryogenic cultures with respe ct to the unchallenged control cultures and the stock plants. The diff erences involved both the absence and the presence of additional RAPD markers in the resistant lines, although the former was most commonly observed. The absence of differences between the unchallenged control of either cultivar and DNA from the leaves of parent trees confirmed t hat the changes were not due to prolonged maintenance in liquid cultur es.