E. Skoglund et al., HIGH-PERFORMANCE CHROMATOGRAPHIC-SEPARATION OF INOSITOL PHOSPHATE ISOMERS ON STRONG ANION-EXCHANGE COLUMNS, Journal of agricultural and food chemistry, 46(5), 1998, pp. 1877-1882
Inositol phosphates are focused on because of their functions in the c
ell, as well as their effects on the bioavailability of certain minera
ls. To improve the separation of inositol hexakis-to monophosphates (I
nsP(6)-InsP(1)) and their positional isomers, six different strong ani
on exchange columns (OmniPac PAX-100, CarboPac PA-100, CarboPac PA-10,
IonPac AS11, Mini Q PC 3.2/3, and ION-120 anion column) were compared
in two ion chromatographic analysis systems. InsP(2)-InsP(6) were aci
dic gradient eluted, postcolumn derivatized, and UV detected in system
1, and InsP(3)-InsP(1) were alkali gradient eluted and detected using
chemically suppressed conductivity detection in system 2. Differences
in retention data were shown depending on the column characteristics
for inositol phosphates with various numbers of phosphate groups attac
hed to the inositol ring. In system 1 the anion exchange columns PA-10
and PA-100 were best suited for the separation of isomers of InsP(2)
and InsP(6)-InsP(3), respectively. Mobile phase optimization was used
to successfully separate inositol phosphate isomers, as illustrated in
a food sample. In system 2, PAX-100 and AS11 were the only columns us
eful to separate isomers of InsP(3)-InsP(1) and InsP(3)-InsP(2), respe
ctively, using the current eluent (NaOH).