A HIGH-RESOLUTION MICROSATELLITE MAP OF THE MOUSE GENOME

The European Collaborative Interspecific Backcross (EUCIB) resource wa s constructed for the purposes of high-resolution genetic mapping of t he mouse genome (Breen et al. 1994). The large Mus spretus/C57BL/6 bac kcross of 982 progeny has a genetic resolution of 0.3 cM at the 95% co nfidence level (similar to 500 kb in the mouse genome). We have used t he EUCIB mapping resource to develop a genome-wide high-resolution gen etic map incorporating 3368 microsatellites. The microsatellites are d istributed among 2302 genetically separated bins with 1.46 markers per bin on average. Average bin separation is 0.61 cM. This high-resoluti on genetic map will aid the construction of a robust physical map of t he mouse genome.