BIOCHEMICAL-CHARACTERIZATION OF BOVINE ALPHA(S1)-CASEIN-F AND GENOTYPING WITH SEQUENCE-SPECIFIC PRIMERS

Bovine alpha(s1)-casein F (alpha(s1)-CN F) was found in a genetic reso urce of Deutsches Schwarzbuntes Niederungsrind cows at a frequency of 0.009. Biochemical characterization of this new variant was obtained b y automated sequencing of reversed-phase HPLC-separated tryptic peptid es of alpha(s1)-CN F and alpha(s2)-CN B. alpha(s1)-CN F was found to b e a subtype of alpha(s1)-CN B with a single aminoacid substitution (Se rP/Leu) in position 66. DNA sequencing revealed a C/T transition in po sition 8418 of the gene. Sequence-specific primers were designed to pe rform an allele-specific polymerase chain reaction for detection of al pha(s1) Cn(F). Typing of artificial insemination sperm samples include d in the genetic resource sperm pool identified one sire heterozygous for alpha(s1) Cn(F).