A polyclonal antibody against a borate-RG-II complex is raised in rabb
its. The antibody recognized RG-II exclusively in cell wall polysaccha
rides, Immunochemical studies demonstrated that the epitope is ubiquit
ous in cell walls of all the cells in radish and rice roots, cultured
tobacco cells, red clover root nodules, and lily growing pollen tubes,
The label was denser in proximal to plasma membrane, and not detected
in middle lamella, suggesting that berate may cross-link newly secret
ed pectic polysaccharides at the membrane-cell wall interface.