IMMUNOCYTOCHEMISTRY OF RHAMNOGALACTURONAN-II IN CELL-WALLS OF HIGHER-PLANTS

A polyclonal antibody against a borate-RG-II complex is raised in rabb its. The antibody recognized RG-II exclusively in cell wall polysaccha rides, Immunochemical studies demonstrated that the epitope is ubiquit ous in cell walls of all the cells in radish and rice roots, cultured tobacco cells, red clover root nodules, and lily growing pollen tubes, The label was denser in proximal to plasma membrane, and not detected in middle lamella, suggesting that berate may cross-link newly secret ed pectic polysaccharides at the membrane-cell wall interface.