Yy. Yeh et al., MODIFICATION OF MILK FORMULA TO ENHANCE ACCRETION OF LONG-CHAIN N-6 AND N-3 POLYUNSATURATED FATTY-ACIDS IN ARTIFICIALLY REARED INFANT RATS, Lipids, 33(5), 1998, pp. 513-520
Artificially reared infant rats were used to determine the effects of
long-chain polyunsaturated fatty acid (LCPUFA) supplementation on bloo
d and tissue concentrations of arachidonic acid (AA) and docosahexaeno
ic acid (DHA). Beginning at 7 d of age, infant rats were fed for 10 d
with rat milk formulas supplemented with AA at 0, 0.5 and 1.0%, or sup
plemented with DHA at 0, 0.5 and 1.0% of total fatty acid. The supplem
entation of AA increased accretion of the fatty acid in tissue and blo
od phospholipids with a maximum increase of 9% in brain, 15% in liver,
25% in erythrocytes, and 43% in plasma above the values of unsuppleme
nted infant rats. Rat milk formula containing 1.0% of AA had no added
benefits over that containing 0.5% of AA. The supplementation of DHA i
ncreased phospholipid DHA by a maximum of 24% in brain, 87% in liver,
54% in erythrocytes, and 360% in plasma above the unsupplemented contr
ol. The increase in tissue and blood DHA was concentration-dependent o
n formula fatty acid. Brain phosphatidylcholine and phosphatidylethano
lamine were similarly enriched with AA and DHA by supplementation of t
he corresponding fatty acids. In general the observed increase of AA w
as accompanied by a decrease in 16:0, 18:1n-9, and/or 18:2n-6, whereas
the increased DHA was associated with a reduction of 18:1n-9, 18:2n-6
, and/or 20:4n-6. Clearly, infant rats were more responsive to DHA tha
n AA supplementation, suggesting a great potential of dietary manipula
tion to alter tissue DHA concentrations. However, the supplementation
of DHA significantly decreased tissue and blood AA/DHA ratios (wt%/wt%
), whereas there was little or no change in the ratio by AA supplement
ation. Although the physiological implications of the levels of AA and
DHA, and AA/DHA ratios achieved under the present experimental condit
ions are not readily known, the findings suggest that artificial reari
ng could provide a suitable model to investigate LCPUFA requirements u
sing various sources of AA and DHA in rats.